What is Rosa26 locus?

Known in scientific circles as Gt(ROSA)26Sor, the Rosa26 locus is a specific genetic site that is located on mouse chromosome 6, and is considered to be one of the ideal locations where knock-ins of interest can be targeted.

What is whole body Knockout?

A constitutive Knockout mouse, also referred to as a conventional or whole-body Knockout (KO), defines a mouse model in which the target gene is permanently inactivated in the whole animal, in every cell of the organism.

What is humanized gene?

Humanized genetically engineered models (GEMs) contain one or more human genes that have been inserted into the mouse genome. This can be achieved through either transgenesis or targeted gene replacement (knock-ins).

Why is ROSA26 used?

The Rosa26 locus is a useful place for inserting a gene, The location of the insertion is known — not random — and it allows scientists to study a gene without affecting the function of other genes. Knock-in models using this locus offer greater accuracy and reproducibility of results.

What is the AAVS1 locus?

The adeno-associated virus site 1 (AAVS1) locus in the human genome is a strong candidate for gene therapy by insertion of an exogenous gene into the locus. The AAVS1 locus includes the coding region for myosin binding subunit 85 (MBS85).

What is Cre knockout?

A Cre switch for transgenes Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression. The proper insertion of a loxP-flanked “stop” sequence (transcriptional termination element) between the promoter and transgene coding sequence blocks the expression of the gene.

What is a knockin mouse?

Knockin mouse models, also called KI mice, are generated to alter a gene sequence by one-for-one substitution with a transgene, or by adding a gene sequence that is not found within the locus.

What are WT mice?

The wild type (WT) is the phenotype of the typical form of a species as it occurs in nature. “Mutant” alleles can vary to a great extent, and even become the wild type if a genetic shift occurs within the population.

How are monoclonal antibodies humanized?

A type of antibody made in the laboratory by combining a human antibody with a small part of a mouse or rat monoclonal antibody. The mouse or rat part of the antibody binds to the target antigen, and the human part makes it less likely to be destroyed by the body’s immune system.

Is Rosa26 a promoter?

The Rosa26 promoter has even been cloned as a bacterial artificial chromosome (BAC), and harnessed to drive ubiquitous expression of other reporter genes in transgenic mice. The Jackson Laboratory currently distributes over 100 mice that have transgenes inserted in the Gt(ROSA)26Sor locus.