What is periodic acid Schiff stain used for?

The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.

How do you make periodic acid Schiff stain?

Procedure:

1. Deparaffinize and hydrate to water.
2. Oxidize in 0.5% periodic acid solution for 5 minutes.
3. Rinse in distilled water.
4. Place in Schiff reagent for 15 minutes (Sections become light pink color during this step).
5. Wash in lukewarm tap water for 5 minutes (Immediately sections turn dark pink color).

What Colour does periodic acid Schiff stain?

red
Periodic acid-Schiff reaction (PAS) The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. This reaction results in a deep red colour in the section. It is the basis of the PAS stain.

How does the PAS stain work?

PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. CONTROL: For staining fungus; use a known positive such as those used for the GMS.

What is periodic acid Schiff with diastase?

Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease.

What stains positive for PAS?

Germ Cell Neoplasms of the Ovary Periodic acid–Schiff (PAS) stain highlights the abundant glycogen in the tumor cells; the staining will disappear after diastase treatment. By immunohistochemistry, dysgerminoma cells are positive for PLAP (strong, cytoplasmic staining), SALL4, CD117, and OCT4 (Fig. 16.7).

What is Schiff reagent formula?

Fuschsine or rosaniline hydrochloride is a magenta-colored dye with a chemical formula C20H20N3·HCl, which is decolorized by sodium bisulfate [1,2].

How long does a PAS stain take?

Procedure for Periodic Acid-Schiff (PAS) Staining Aldehydration: Place the stain in Schiff reagent for 15 minutes, which turns light pink. Washing: Using lukewarm tap water, wash the stain for 5 minutes, turning it dark pink.

What stains blue with H&E stain?

H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors.

How long does PAS staining take?

Rinsing: In distilled water. Aldehydration: Place the stain in Schiff reagent for 15 minutes, which turns light pink. Washing: Using lukewarm tap water, wash the stain for 5 minutes, turning it dark pink.

What is periodic acid Schiff reagent?

Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. A suitable basic stain is often used as a counterstain.

What does a PAS stain look like?

PAS-D is a stain often used by pathologists as an ancillary study in making a histologic diagnosis on paraffin-embedded tissue specimens. PAS stain typically gives a magenta color in the presence of glycogen. When PAS and diastase are used together, a light pink color replaces the deep magenta.