What is pBI121?

The vector pBI121 is 14758 bp in length and the GenBank accession number is AF485783. The segment of 162 bp (positions 2357 to 2518 in complement strand) including the central 25-bp repeat (positions 2454 to 2478 in complement strand) is known as the T-DNA right border region.

What is pcambia1301?

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GUS genes.

What is meant by binary vector?

A binary vector is a standard tool in the transformation of higher plants mediated by Agrobacterium tumefaciens. It is composed of the borders of T-DNA, multiple cloning sites, replication functions for Escherichia coli and A. A number of useful vectors are widely circulated.

What is NOS promoter?

The nos promoter is extensively used in plant transformation vectors to drive the expression of selectable marker genes. Promoter activity has been found to increase during flower development and expression in anthers, using cat as a reporter gene, has been noted (An et al, 1988).

What is pcambia2301?

Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes.

Is pCAMBIA a binary vector?

pCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection gene, which can expdrssed in plant system very well.

Why are binary vectors called binary?

They are artificial vectors that have been derived from the naturally occurring Ti plasmid found in bacterial species of the genus Agrobacterium, such as A. The binary vector is a shuttle vector, so-called because it is able to replicate in multiple hosts (e.g. Escherichia coli and Agrobacterium).

What is the basis of binary vector strategy?

What is the basis of “binary vector” strategy? Explanation: The binary vector strategy is based on the observation that the T-DNA does not need to be physically present/attached to the rest of the Ti plasmid.

What is nptII gene?

The nptII gene codes for an aminoglycoside phosphotransferase conferring resistance to antibiotics such as kanamycin, neomycin, paromomycin, butirosin, gentamycin B and geneticin.

How are expression vectors useful?

The vector is used to introduce a specific gene into a target cell, and can commandeer the cell’s mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.

What is a Bacmid Wikipedia?

Noun. bacmid (plural bacmids) (cytology) A shuttle vector that can be propagated in both E. coli and insect cells.