Is Zeocin same as bleomycin?

Zeocin™ is a member of the bleomycin/phleomycin family of antibiotics isolated from Streptomyces.

What is Zeocin used for?

Usage. Zeocin and other related chemicals in the bleomycin family of compounds are primarily used in molecular biology as an antibiotic, especially for the selection of eukaryotic cell lines when used in conjunction with vectors containing a selectable marker for zeocin resistance.

How long does Zeocin take to kill cells?

Add medium with varying Zeocin™ concentrations (0, 50,100, 200, 400, 600, 800, and 1000 μg/ml) to each plate. Replenish selective medium every 3-4 days and observe the percentage of surviving cells. Select the concentration that kills the majority of cells within 1-2 weeks.

Does Zeocin expire?

Plates containing Zeocin® are stable for 1 month when stored at 4 °C.

What is blasticidin resistance?

Blasticidin S is a microbial antibiotic that inhibits protein synthesis in both prokaryotes and eukaryotes. The blasticidin S-resistance gene (bsr), isolated from Bacillus cereus K55-S1 strain, was inserted into pSV2 plasmid vector and introduced into cultured mammalian cells by transfection.

What is BleoR?

BleoR is an Sh ble from Streptoalloteichus hindustanus, encoding antibiotic-binding protein, confers resistance to bleomycin, phleomycin, and Zeocin. Here is a part named BleoR on MolecularCloud.

How do you make Zeocin?

Making the stock solution: Weigh out 1 g of ZeocinTM (Phleomycin, Gold Biotechnology, Catalog #Z-200 [CAS 11006-33-0, MW: 1526.5]). 2. Add 10 ml of sterile H2O, or Hepes Buffer (5 g/L, pH 7.25). Dissolve completely by mixing or vortexing.

How do you make Zeocin stock solution?

How do you dilute blasticidin?

Preparing and Storing Stock Solutions

1. Prepare Blasticidin stock solutions of 5-10 mg/ml by dissolving Blasticidin in sterile water and filter-sterilize the solution.
2. Aliquot in small volumes suitable for one time use and store at 4ºC (short term) or at -20ºC (long-term).

How fast is blasticidin selection?

Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week.