What is a heparin column?

What is a heparin column?

HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins. Useful for screening and optimization before scaling up to bulk resins such as Heparin 6 Fast Flow.

What is heparin affinity chromatography?

Heparin chromatography is an adsorption chromatography in which biomolecules can be specifically and reversibly adsorbed by heparins immobilized on an insoluble support. An advantage of this chromatography is that heparin-binding proteins can be conveniently enriched using its concentration effect.

How do you clean heparin columns?

Cleaning. Remove ionically bound proteins by washing with 0.5 column volume 2 M NaCl for 10–15 minutes. Remove precipitated or denatured proteins by washing with 4 column volumes 0.1 M NaOH for 1–2 hours or 2 column volumes 6 M guanidine hydrochloride for 30–60 minutes or 2 column volumes 8 M urea for 30–60 minutes.

What is heparin sepharose?

Abcam’s Heparin Sepharose® is designed for purification of heparin-binding proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient.

What type of protein is heparin?

Heparin is a highly sulfated form of HS that is made predominantly by connective tissue mast cells as a large heparin proteoglycan (750–1000 kDa) consisting of a small core protein, serglycin, with multiple heparin polysaccharide chains (1,13).

How do you regenerate a heparin column?

Regenerate the column with 2-3 bed volumes of 8 M urea, 1.5 M NaCl in PBS. Follow the urea wash with 3-5 bed volumes of application buffer. The column is now ready for re-use.

How is heparin manufactured?

Pharmaceutical-grade heparin is derived from mucosal tissues of slaughtered meat animals such as porcine (pig) intestines or bovine (cattle) lungs. Advances to produce heparin synthetically have been made in 2003 and 2008.

What is the main function of heparin?

Heparin injection is an anticoagulant. It is used to decrease the clotting ability of the blood and help prevent harmful clots from forming in blood vessels. This medicine is sometimes called a blood thinner, although it does not actually thin the blood.