What does nopaline synthase do?

What does nopaline synthase do?

Nopaline synthase (nos) is of bacterial origin and expressed in plant tissues to generate an unusual compound, nopaline, which is secreted into the environment where Agrobacterium tumefaciens uses it as a nutrient source [9].

Where does nos terminator come from?

For nearly 30 years, the most widely used terminators have been the nopaline synthase (NOS) and octopine synthase (OCS) terminators from Agrobacterium tumefaciens, and the 35S terminator from cauliflower mosaic virus (MacFarlane et al., 1992; Ellis et al., 1987; Pietrzak et al., 1986).

What is nos terminator?

A transcription terminator – usually just called a terminator – is a DNA sequence that marks the end of a gene for transcription. The NOS terminator is a 127 bp sequence found in genetically modified plants. In addition to their own chromosomes, plants contain organelles with DNA called chloroplasts.

What is nopaline synthase gene?

Genetic element (GENE) Nopaline synthase is an enzyme which catalyses the synthesis of nopaline, an opine which is formed as the result of the condensation of the amino acid arginine and alpha-ketoglutaric acid. The type of opine produced is specific to the particular strain of A. tumefaciens.

What is octopine and nopaline?

Nopaline is a chemical compound derived from the amino acids glutamic acid and arginine. These may be nopaline plasmids, octopine plasmids and agropine plasmids. These opines are condensation products of amino acids and keto acids or may be derived from sugars.

Is the terminator the same as a stop codon?

A terminator sequence is a nucleic acid sequence that causes RNA polymerase to stop its activity. It marks the end of a gene or operon, therefore stopping transcription. A stop codon is an mRNA nucleotide triplet that signals the end of the newly formed polypeptide chain during translation.

What is 35S promoter and NOS terminator?

In this study, based on the regulatory sequence of the cauliflower mosaic virus 35S (CaMV-35S) promoter and the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (GM) crops, we designed two sets of RPA primers and established a real-time RPA …

What is the meaning of Octopine?

Octopine meaning (chemistry) An analog of lactic acid, derived from the amino acids arginine and alanine, first isolated from the muscle tissue of octopus species. noun. Of or pertaining to an octopus or octopuses.

Who discovered plasmid?

Braun demonstrated that the incitement of crown galls by Agrobacteriurn requires a wounding of plants and subsequent wound healing for at least 24 to 48 h. Furthermore, he found in 1947 that the exposure of plants to 32 “C at any time within four to five days after Agrobacterium infection inhibits tumor formation.