What is Susa fixative?

A general-purpose histological fixative that contains mercuric chloride.

How do you make heidenhain Susa?

Heidenhain “Susa”composition:

1. HgCl2 -45gm.
2. Nacl – 5gm.
3. Formalin (40% formaldehyde solution )- 200 ml.
4. Glacial acetic acid – 40 ml.
5. Trichloroacetic acid – 20 gm.
6. Distilled water – 800 ml.

What is Zenker’s fluid used for?

A tissue fixative consisting of mercuric chloride, potassium dichromate, glacial acetic acid, and water. It is used to examine cells, and particularly nuclei, in detail.

Which fixative is preferred mostly for histochemical study?

Crosslinking fixatives – aldehydes The most commonly used fixative in histology is formaldehyde. It is usually used as a 10% neutral buffered formalin (NBF), that is approx. 3.7%–4.0% formaldehyde in phosphate buffer, pH 7.

Which fixative is likely to cause violent explosions?

Picric acid is highly explosive when dry, and therefore must be kept moist with distilled water or saturated alcohol at 0.5 to 1% concentration during storage.

Which is ideal fixative for fixing chromosomes?

The best fixative is redistilled glutaraldehyde; even so, although it leaves the nucleus visually unaltered, a network forms in the nuclear sap and birefringence is lost. Calcium ions in the fixative cause chromosomal movements. The fixatives may alter nucleoli and may dissolve them completely.

How do you make Zenker’s solution?

Stock solution Zenker is usually made with 50g of mercuric chloride, 25g of potassium dichromate, 10g of sodium sulfate (decahydrate) and distilled water to complete 1000 ml. Before use, 5 ml glacial acetic acid is added to 100 ml of the solution.

How do you make Zenker’s fluid?

Zenker’s fluid fixation

1. Add 950ml distilled water to a suitable container.
2. Add 25g potassium dichromate.
3. Add 50g mercuric chloride.
4. Add 50g glacial acetic acid.
5. Mix thoroughly to dissolve.
6. Fix samples for 4-24 hours.
7. Following fixation wash samples overnight in running tap water prior to processing.

Which fixative permanently fix lipids?

Osmium tetroxide It is soluble in polar and non-polar solvents and reacts with the side chains of proteins forming cross-links. The most important fixation reactions of osmium tetroxide are those involving unsaturated bonds of lipids and phospholipids as it is one of the few fixatives that stabilises lipids.

Which is the most commonly used fixative in clinical practice?

1. Phosphate buffered formalin. The most widely used formaldehyde-based fixative for routine histopathology. The buffer tends to prevent the formation of formalin pigment.

What is the most rapid fixative?

Formaldehyde is commonly used as 4% solution, giving 10% formalin for tissue fixation. Formalin is most commonly used fixative. It is cheap, penetrates rapidly and does not over- harden the tissues. The primary action of formalin is to form additive compounds with proteins without precipitation.

Which fixative is explosive?

Picric acid
Picric acid (2,4,6-trinitrophenol) is a highly explosive compound most commonly used in military applications.