How do you make antibiotic stock solutions?

How do you make antibiotic stock solutions?

Preparation of 80ml stock solution

  1. Tetracycline is kept in the 4C fridge in 68-564D.
  2. Weigh 400mg of tetracyline HCL into a small weigh boat.
  3. Dilute 95% Ethanol to 70% using milliQ water.
  4. Add 80ml of 70% Ethanol to a 250ml bottle.
  5. Add the tetracycline HCL to the ethanol.

Is chloramphenicol soluble in water?

Solubility : Soluble in water (2.5 g/l) at 25° C, methanol, ethanol, butanol, ethyl acetate, acetone, DMSO, and propylene glycol (150.8 mg/ml).

What is the molecular weight of chloramphenicol?

323.132 g/mol
Chloramphenicol/Molar mass

What is stock solution microbiology?

In chemistry, a stock solution is a large volume of common reagent, such as hydrochloric acid or sodium hydroxide, at a standardized concentration. In biochemistry, the term is often used to refer to a concentrated solution, from which one can dilute into a working concentration of solution.

How do you make amoxicillin stock solution?

Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.

What is chloramphenicol?

Chloramphenicol is a semisynthetic, broad-spectrum antibiotic derived from Streptomyces venequelae with primarily bacteriostatic activity. Chloramphenicol diffuses through the bacterial cell wall and reversibly binds to the bacterial 50S ribosomal subunit.

Why is chloramphenicol used for?

Chloramphenicol is an antibiotic. It’s mainly used to treat eye infections (such as conjunctivitis) and sometimes ear infections. Chloramphenicol comes as eye drops or eye ointment. These are available on prescription or to buy from pharmacies.

What is stock solution preparation?

A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume.

What is meant by Standard stock solution?

A standard solution or a stock solution is a solution whose concentration is accurately known. At the time of experiment, the solution with required concentration is prepared by diluting the stock solution. This diluted solution is called working standard.