What is the theory of HPLC?

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.

What is quantification in HPLC?

Quantitative results in high-performance liquid chromatography (HPLC) are usually based on calibration with standards and peak-area measurement. A distinctive method to determine the absolute number of moles of a com- pound from peak-area measurements using HPLC with a UV–vis detector has been proposed (1,2).

What is liquid chromatography nuclear magnetic resonance?

Abstract. The direct coupling of high-performance liquid chromatography (HPLC) to nuclear magnetic resonance (NMR) has evolved as a powerful new tool (HPLC/NMR) for the analysis of mixtures. An inherent advantage of HPLC/NMR is the ability to monitor the NMR chemical shift dimension.

What is K value in HPLC?

The retention (or capacity) factor (k) is a means of measuring the retention of an analyte on the chromatographic column. Determination of Retention Factor (k) A high k value indicates that the sample is highly retained and has spent a significant amount of time interacting with the stationary phase.

What are the different types of HPLC?

Different Types of HPLC Columns Used in Analysis

• Normal Phase HPLC Columns: This type of columns has more polar stationary phase than the mobile phase.
• Reverse Phase HPLC Columns:
• Ion Exchange HPLC Columns:
• Size Exclusion HPLC Columns:

Which is used in NMR spectroscopy?

The sample is placed in a magnetic field and the NMR signal is produced by excitation of the nuclei sample with radio waves into nuclear magnetic resonance, which is detected with sensitive radio receivers.

What is void volume in HPLC?

The HPLC column void volume denoted Vm or V0 is in simple terms the volume of the mobile phase in the column. It is the part of a fraction that when added to the volume of the stationary phase makes up a whole fraction or 100% volume.

What is cyano column?

Answer: Cyano columns are usually prepared by bonding a cyanopropyl silane to the silica. This results in a column that has some polar functionality due to the CN group and some nonpolar functionality due to the propyl group.