What is the difference between UV-Vis spectroscopy and IR spectroscopy?

IR spectroscopy gives a good indication of the functional groups present in a molecule. UV-vis spectroscopy is a much more quantitative technique.

What is the range of UV visible and IR spectroscopy?

Ultraviolet-Visible-near-IR Spectroscopy (UV-Vis-NIR)​ Measurements in the ultraviolet/visible region (UV-VIS) cover wavelengths from about 200 nm to 800 nm. The absorption of ultraviolet or visible radiation by a molecule leads to transitions among the electronic energy levels of the molecule.

How UV visible spectroscopy is different from IR spectroscopy in polymer chemistry?

Unlike infrared spectra, UV–Vis spectra are quite broad and generally show only a small number of peaks. The peaks are reported as the wavelengths where maxima occur.

How does UV-VIS NIR spectroscopy work?

Ultraviolet-visible (UV-Vis) spectrophotometers use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm). The instruments then measure the light absorbed, transmitted, or reflected by the sample at each wavelength.

Why UV-Vis spectra is broader than IR spectra?

In UV-Visible spectra Bonds will be in constant vibration, this variation will absorb nearby energies i.e, ΔE , for this reason UV peaks are broader. Spectrum is broaden by spontaneous emission.

How do you perform a UV-VIS spectroscopy?

1. Fill the cuvette with the sample.
2. Place the cuvette in the spectrometer in the correct direction.
3. Cover the cuvette to prevent any ambient light.
4. Collect an absorbance spectrum by allowing the instrument to scan through different wavelengths and collect the absorbance.

Why the detector used in UV spectrometer are not used in IR spectrometer?

As IR radiation is weak energy radiation it can not cause, damage or lysis of analyte, as UV radiation (high energy) may cause. So no need to mount absorption monochromator in between IR Source and Sample.

How do you do UV-Vis spectroscopy?

Where is UV-Vis spectroscopy?

Ultraviolet-visible (UV-Vis) spectroscopy is a widely used technique in many areas of science ranging from bacterial culturing, drug identification and nucleic acid purity checks and quantitation, to quality control in the beverage industry and chemical research.

Why is UV-Vis spectroscopy used?

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and certain biological macromolecules. Measurement is usually carried out in solution.