Users' questions

What does oroboros measure?

What does oroboros measure?

Oroboros O2K- is a state-of-the art respirometer for quantitative high-resolution respirometry and comprehensive OXPHOS analysis and combination of respirometry with fluorometry for simultaneous real-time monitoring of ROS production, mt- membrane potential, ATP production and Ca2+.

Which of the following method is used to measure respiratory efficiency of cells in cell culture?

Extracellular flux (XF) analysis has become a mainstream method to measure bioenergetic function in cells and tissues. While this technique is commonly used to measure energetics in intact cells, we outline here a detailed XF protocol for measuring respiration in permeabilized cells.

What is high resolution Respirometry?

High-resolution respirometry, HRR, is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of mitochondrial preparations and living cells combined with MultiSensor modules.

How does an Oxygraph work?

The high-resolution oxygraph device is equipped with two chambers and uses polarographic oxygen sensors to measure oxygen concentration and calculate oxygen consumption within each chamber. Oxygen consumption rates are calculated using software and expressed as picomoles per second per number of cells.

What is ouroboros dragon?

The ouroboros or uroboros (/ˌjʊərəˈbɒrəs/) is an ancient symbol depicting a serpent or dragon eating its own tail. The ouroboros is often interpreted as a symbol for eternal cyclic renewal or a cycle of life, death, and rebirth.

What does viable cells mean?

Cell viability is a measure of the proportion of live, healthy cells within a population. Typically, cell viability assays provide a readout of cell health through measurement of metabolic activity, ATP content, or cell proliferation.

How do you increase mitochondrial respiration?

Strategies to Improve Mitochondrial Function

  1. Pick the right mother.
  2. Optimize nutrient status to limit oxygen and high-energy electron leakage in the ETC.
  3. Decrease toxin exposure.
  4. Provide nutrients that protect the mitochondria from oxidative stress.
  5. Utilize nutrients that facilitate mitochondrial ATP production.

Why are mitochondria so important?

As the power plants in virtually every human cell (as well as animal, plant, and fungi cells), mitochondria play an essential role in creating energy to drive cellular function and basically all of our biological processes.

What is oxygen electrode?

An electrode used to measure the partial pressure of oxygen (Po2) or the blood glucose concentration of a fluid sample. Synonym: Clark electrode; Po2 electrode; polarographic electrode.